Genotypic analysis of Mycobacterium tuberculosis from medieval human remains

Genotypic analysis of Mycobacterium tuberculosis from medieval human remains.

Three medieval bone samples with osteological evidence of tuberculosis infection were analysed for the presence of DNA sequences from Mycobacterium tuberculosis using a series of PCRs. In each case amplification of IS6110 and part of the beta-subunit of RNA polymerase identified infection with a bacterium belonging to the M. tuberculosis complex. Amplification of the mtp40 genome fragment and t...

Study of Preservation Status and Dietary Reconstruction in Human Remains Recovered from Roopkund Lake through Chemical Analysis of Faunal Remains

The Present study carried out on the bone samples collected from Roopkund Lake in Chamoli Garhwal, Uttarakhand, which is located 5029 meters from main sea level in between Nanda Ghunghti and Trishuli peak. This historical site belongs to the 9th century A.D.  All the samples selected for the study were dried in room temperature, as well as in hot air ovens at 32 degree Celsius. The Cleaning, pr...

Phenotypic and Genotypic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Isolates from Sudanese Patients

Background. Currently, mutations in rpoB, KatG, and rrs genes and inhA promoter were considered to be involved in conferring resistance to rifampicin, isoniazid, and streptomycin in Mycobacterium tuberculosis (MTB). Objective. The aims of this study were to detect the prevalence of first-line tuberculosis (TB) drug resistance among a group of previously treated and newly detected TB patients, t...

Genotypic analysis of Mycobacterium tuberculosis isolates from a Lisbon hospital in Portugal.

Portugal has one of the highest tuberculosis notification rates of the European Union with Lisbon Health Region having an incidence rate well above the national average. The present study analyses the transmission, drug susceptibility and characteristics of a study population from a Central Lisbon's Hospital. One hundred and thirty -two Mycobacterium tuberculosis clinical isolates were previous...

Extraction and Analysis of Ancient DNA from Human Remains of Masjede Kabood Burial Site